goat anti mouse lgg fitc  (Boster Bio)

Journal: Science Advances

Article Title: In situ coagulation environment regulation–assisted thrombus clearance via hydrogenated silicon-based nanothrombolytics

doi: 10.1126/sciadv.aea4782

Figure Lengend Snippet: ( A ) Preparation procedure and antithrombus mechanism of SiH@UK/Fib. ( B ) TEM result and element mapping of SiH NS. ( C ) AFM images of SiH and SiH@UK/Fib. ( D ) Thickness distribution of the SiH and SiH@UK/Fib quantified from (C). ( E ) Hydrodynamic diameter distributions of SiH@Fib after treated with thrombin for different time periods. ( F ) SEM results of the thrombus in 10-min incubation with SiH@Fib in PBS and plasma, respectively. ( G ) Confocal image of tetramethyl rhodamine isothiocynate (TRITC)–labeled thrombus (red) in 10-min incubation with fluorescein isothiocyanate (FITC)–labeled SiH@Fib.

Article Snippet: Rabbit anti-Nrf2 antibody, rabbit anti–p-Nrf2 antibody, rabbit anti-CD41 antibody, rabbit anti–PAI-1 antibody, rabbit anti–collagen I antibody, FITC-labeled goat anti-rabbit immunoglobulin G (IgG) antibody, and Cy3-labeled goat anti-rabbit IgG antibody were obtained from Bioss Biotechnology Co. Ltd. (Beijing, China).

Techniques: Incubation, Clinical Proteomics, Labeling

Journal: bioRxiv

Article Title: Differential Assembly of Native ENaC Complexes Across Mouse Epithelial Tissues

doi: 10.64898/2026.01.23.701393

Figure Lengend Snippet: a . Schematic of the tagged ENaCγ subunit produced from the ENaCγ-VF allele, which includes a C-terminal mVenus, 3C protease site, and 3xFLAG tag. b . Representative immunofluorescence image showing co-localization of mVenus and ENaCγ in kidney tissue. mVenus signal was detected using a FITC-conjugated anti-GFP primary antibody (goat, Rockland, 1:100) and Alexa Fluor 488 donkey anti-goat secondary antibody. Endogenous ENaCγ was detected using a rabbit anti-ENaCγ primary antibody (StressMarq, 1:500) targeting the C-terminus, and Alexa Fluor 647 donkey anti-rabbit secondary antibody. DAPI is 4’,6-diamidino-2-phenylindole . c . Close-up view highlighting localization of tagged ENaCγ.

Article Snippet: Endogenous ENaCγ was detected using a rabbit anti-ENaCγ primary antibody (StressMarq, 1:500) targeting the C-terminus, and Alexa Fluor 647 donkey anti-rabbit secondary antibody.

Techniques: Produced, Immunofluorescence

Journal: bioRxiv

Article Title: Differential Assembly of Native ENaC Complexes Across Mouse Epithelial Tissues

doi: 10.64898/2026.01.23.701393

Figure Lengend Snippet: a. Cryo-EM map of human ENaC in complex with two Fabs, 10D4 and 7B1, shown in side and top-down views. In the top-down view, a red box highlights the interaction site between 7B1 and the α subunit. b. Sequence alignment of the experimentally determined 7B1 epitope region in human and mouse ENaCα subunits, revealing high conservation and supporting the potential for cross-reactivity with mouse ENaC. c . Schematic representation of the 7B1-mScarlet Fab construct used to detect the ENaC α subunit. The variable regions of the 7B1 heavy and light chains were expressed as a Fab fragment in HEK293 cells, with mScarlet fused to the C-terminus of the heavy chain. d . FSEC trace monitoring mVenus fluorescence from lung lysates of ENaCγ-VF mice, showing the elution profile of γ-containing ENaC complexes. Dotted lines indicate the elution range for mVenus-tagged complexes (11-16 mL). e . FSEC trace from the same sample following 7B1-mScarlet fluorescence, revealing the elution profile of α-containing ENaC complexes. Dashed lines show that the α-associated signal is restricted to a narrower elution range (11-13 mL), suggesting that α-γ co-assemblies represent a more defined subset of the total γ-containing complexes. f . Overlay of the FSEC traces shown in panels d and e , plotted on the same axes to facilitate direct comparison of elution profiles and peak positions. The inset shows the full normalized traces for each condition. The main panel displays a magnified view of the gray-shaded region indicated in the inset, highlighting the relative alignment of the major peaks.

Article Snippet: Endogenous ENaCγ was detected using a rabbit anti-ENaCγ primary antibody (StressMarq, 1:500) targeting the C-terminus, and Alexa Fluor 647 donkey anti-rabbit secondary antibody.

Techniques: Cryo-EM Sample Prep, Sequencing, Construct, Fluorescence, Comparison